喜洋洋公式规律论坛
抗體 > 多克隆抗體 > HIST1H3A (Ab-17) Antibody
  • 產品類型:
    Polyclonal Antibody
  • 產品名稱:

    HIST1H3A (Ab-17) Antibody

  • 貨號:
    CSB-PA010418OA17nme1HU
  • 規格:
    ¥2200
  • 別名:
    Histone H3.1 (Histone H3/a) (Histone H3/b) (Histone H3/c) (Histone H3/d) (Histone H3/f) (Histone H3/h) (Histone H3/i) (Histone H3/j) (Histone H3/k) (Histone H3/l), HIST1H3A; HIST1H3B; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J, H3FA; H3FL; H3FC; H3FB; H3FD; H3FI; H3FH; H3FK; H3FF; H3FJ
  • 種屬:
    Human
  • 免疫原:
    Peptide sequence around site of Arg (17) derived from Human Histone H3.1
  • 宿主:
    Rabbit
  • 種屬反應性:
    Human, Mouse
  • 應用范圍:
    ELISA, WB, IHC, IF, ChIP; Recommended dilution: WB:1:200-1:2000, IHC:1:20-1:200, IF:1:50-1:200
  • 背景:
    Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
  • 克隆類型:
    Polyclonal
  • 抗體亞型:
    IgG
  • 純化方式:
    Antigen Affinity Purified
  • 標記方式:
    Non-conjugated
  • 保存緩沖液:
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 產品提供形式:
    Liquid
  • 儲存條件:
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 圖片:


    Western Blot

    Positive WB detected in: NIH/3T3 cell acid extracts, Hela cell acid extracts, A549 cell acid extracts, PC-3 cell acid extracts, HEK293 cell acid extracts

    All lanes: HIST1H3A antibody at 1.6µg/ml

    Secondary

    Goat polyclonal to rabbit IgG at 1/50000 dilution

    Predicted band size: 16 kDa

    Observed band size: 16 kDa



    Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA010418OA17nme1HU at dilution of 1:100



    Immunohistochemistry of paraffin-embedded human lung cancer using CSB-PA010418OA17nme1HU at dilution of 1:100



    Immunofluorescent analysis of Hela cells using CSB-PA010418OA17nme1HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)



    Chromatin Immunoprecipitation Hela (4*106) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 8µg anti-HIST1H3A (CSB-PA010418OA17nme1HU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.

  • 說明書:
  • Accession No.:
  • 指標名稱:
    HIST1H3A
  • 研究領域:
    Epigenetics and Nuclear Signaling