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試劑盒 > 酶聯免疫試劑盒 > 小鼠載脂蛋白E(Apo-E)ELISA Kit
    • ELISA Kit
    • 小鼠載脂蛋白E(Apo-E)ELISA Kit
    • Chylomicron remnants and very low density lipoprotein (VLDL) remnants are rapidly removed from the circulation by receptor-mediated endocytosis in the liver. Apolipoprotein E, a main apoprotein of the chylomicron, binds to a specific receptor on liver cells and peripheral cells. ApoE is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. The APOE gene is mapped to chromosome 19 in a cluster with APOC1 and APOC2. Defects in apolipoprotein E result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants.[provided by RefSeq]
    • UniProtP08226
    • HGNC613
    • RGD2138
    • MGI88057
    • OMIM107741
    • CUSABIOCSB-E09750m
  • 產品類型:
    ELISA Kit
  • 產品名稱:

    小鼠載脂蛋白E(Apo-E)ELISA Kit

  • 英文名稱:

    Mouse apolipoprotein E (Apo-E) ELISA Kit

  • 貨號:
    CSB-E09750m
  • 別名:
    AD2, LDLCQ5, LPG, MGC1571, apolipoprotein E3
  • 規格:
    96T
  • 中文價格:
    3800
  • 種屬:
    Mus musculus (Mouse)
  • 其他種屬:
  • 待測物名稱:
    apolipoprotein E
  • 縮寫:
    APOE
  • 蛋白功能1:
    Apolipoprotein
  • 蛋白功能3:
    Lipid transport
  • 檢測范圍:
    6.25 ng/mL-400 ng/mL
  • 靈敏度:
    1.56 ng/mL
  • 反應時間:
    1-5h
  • 所需樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 下載:
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    400 0.157 0.159 0.158    
    200 0.249 0.269 0.259    
    100 0.432 0.441 0.437    
    50 0.643 0.672 0.658    
    25 0.955 0.984 0.970    
    12.5 1.199 1.172 1.186    
    6.25 1.568 1.572 1.570    
    0 2.573 2.591 2.582    
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse Apo-E in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:100 Average % 99  
    Range % 96-101  
    1:200 Average % 85  
    Range % 81-89  
    1:400 Average % 100  
    Range % 97-103  
    1:800 Average % 83  
    Range % 80-86  
  • 回收率:
    The recovery of mouse Apo-E spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 97 93-100  
    EDTA plasma (n=4) 99 95-104  
                 
                 
  • 定性/定量:
    quantitative
  • 研究領域:
    Cardiovascular
  • 引用文獻:
    1. Serum levels of 9α, 11β┬GF2 and apolipoprotein A1 achieve high predictive power as biomarkers of anaphylaxis , Wittenberg M.et al,Allergy.,2017