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試劑盒 > 酶聯免疫試劑盒 > 小鼠葉酸(FA)ELISA Kit
    • ELISA Kit
    • 小鼠葉酸(FA)ELISA Kit
  • 產品類型:
    ELISA Kit
  • 產品名稱:

    小鼠葉酸(FA)ELISA Kit

  • 英文名稱:

    Mouse Folic acid,FA ELISA Kit

  • 貨號:
    CSB-E08758m
  • 別名:
    N/A
  • 規格:
    96T
  • 中文價格:
    3800
  • 種屬:
    Mus musculus (Mouse)
  • 其他種屬:
  • 待測物名稱:
    Folic acid,FA
  • 縮寫:
    FA
  • 蛋白功能1:
    Metabolism
  • 檢測范圍:
    0.195 ng/mL-50 ng/mL
  • 靈敏度:
    0.195 ng/mL
  • 反應時間:
    1-5h
  • 所需樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 下載:
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    ng/ml OD1 OD2 Average      
    50 0.321 0.332 0.327      
    12.5 0.512 0.537 0.525      
    3.12 0.826 0.851 0.839      
    0.78 1.175 1.153 1.164      
    0.195 1.655 1.632 1.644      
    0 1.985 1.948 1.967      
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse FA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
      Sample Serum(n=4)    
    1:1 Average % 97    
    Range % 90-105    
    1:2 Average % 89    
    Range % 83-96    
    1:4 Average % 92    
    Range % 84-99    
    1:8 Average % 91    
    Range % 85-99    
  • 回收率:
    The recovery of mouse FA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 95 87-100    
    EDTA plasma (n=4) 95 89-99    
                   
                   
  • 定性/定量:
    quantitative
  • 研究領域:
    Metabolism
  • 引用文獻:
    1. Folic acid prevents cardiac dysfunction and reduces myocardial fibrosis in a mouse model of high-fat diet-induced obesity , Li W.et al,Nutr Metab (Lond),2017