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試劑盒 > 酶聯免疫試劑盒 > 小鼠載脂蛋白B(APOB) ELISA kit
    • ELISA Kit
    • 小鼠載脂蛋白B(APOB) ELISA kit
  • 產品類型:
    ELISA Kit
  • 產品名稱:

    小鼠載脂蛋白B(APOB) ELISA kit

  • 英文名稱:

    Mouse Apolipoprotein B(APOB) ELISA kit

  • 貨號:
    CSB-EL001918MO
  • 規格:
    96T
  • 中文價格:
    3600
  • 種屬:
    Mus musculus (Mouse)
  • 其他種屬:
  • 待測物名稱:
    Apolipoprotein B
  • 縮寫:
    APOB
  • 蛋白功能1:
    Apolipoprotein
  • 蛋白功能2:
    Lipogenesis and lipometabolism
  • 蛋白功能3:
    Cholesterol metabolism
  • 檢測范圍:
    0.156 μg/mL-10 μg/mL
  • 靈敏度:
    0.039 μg/mL
  • 反應時間:
    1-5h
  • 所需樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 下載:
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μg/ml OD1 OD2 Average    
    10 0.091 0.092 0.092    
    5 0.136 0.132 0.134    
    2.5 0.236 0.229 0.233    
    1.25 0.360 0.368 0.364    
    0.625 0.539 0.524 0.532    
    0.312 0.877 0.927 0.902    
    0.156 1.334 1.286 1.310    
    0 2.562 2.747 2.655    
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse APOB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:100 Average % 102  
    Range % 97-108  
    1:200 Average % 103  
    Range % 97-106  
    1:400 Average % 92  
    Range % 88-97  
    1:800 Average % 86  
    Range % 82-91  
  • 回收率:
    The recovery of mouse APOB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 94 89-98  
    EDTA plasma (n=4) 105 102-109  
                 
                 
  • 定性/定量:
    quantitative
  • 研究領域:
    Cardiovascular
  • 引用文獻:
    1. Attenuation of atherogenic apo B-48-dependent hyperlipidemia and high density lipoprotein remodeling induced by vitamin C and E combination and their beneficial effect on lethal ischemic heart disease in mice , S. Contreras-Duarte.et al,Biological Research,2018
    2. Disentangling Host-Microbiota Regulation of Lipid Secretion by Enterocytes: Insights from Commensals Lactobacillus paracasei and Escherichia coli , Asmaa Tazi.et al,American Academy of Microbiology,2018