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試劑盒 > 酶聯免疫試劑盒 > 人載脂蛋白B100(Apo-B100)ELISA Kit
    • ELISA Kit
    • 人載脂蛋白B100(Apo-B100)ELISA Kit
    • This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels.[provided by RefSeq]
    • UniProtP04114
    • BIOWWWAPOB
    • HGNC603
    • RGD2129
    • MGI88052
    • OMIM107730
    • CUSABIOCSB-E08100h
  • 產品類型:
    ELISA Kit
  • 產品名稱:

    人載脂蛋白B100(Apo-B100)ELISA Kit

  • 英文名稱:

    Human apolipoprotein B100 (Apo-B100) ELISA Kit

  • 貨號:
    CSB-E08100h
  • 別名:
    FLDB, LDLCQ4, apoB-100|apoB-48|apolipoprotein B|apolipoprotein B48
  • 規格:
    96T
  • 中文價格:
    3600
  • 種屬:
    Homo sapiens (Human)
  • 其他種屬:
  • 待測物名稱:
    apolipoprotein B (including Ag(x) antigen)
  • 縮寫:
    APOB
  • 蛋白功能1:
    Apolipoprotein
  • 蛋白功能2:
    Lipogenesis and lipometabolism
  • 蛋白功能3:
    Cholesterol metabolism
  • 檢測范圍:
    78 ng/mL-5000 ng/mL
  • 靈敏度:
    19.5 ng/mL
  • 反應時間:
    1-5h
  • 所需樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 下載:
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    5000 0.074 0.073 0.074    
    2500 0.118 0.121 0.120    
    1250 0.182 0.172 0.177    
    625 0.272 0.284 0.278    
    312 0.451 0.442 0.447    
    156 0.770 0.754 0.762    
    78 1.122 1.054 1.088    
    0 2.371 2.270 2.321    
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human Apo-B100 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:200 Average % 96  
    Range % 92-102  
    1:400 Average % 95  
    Range % 91-99  
    1:800 Average % 104  
    Range % 102-108  
    1:1600 Average % 87  
    Range % 81-92  
  • 回收率:
    The recovery of human Apo-B100 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 92-102  
    EDTA plasma (n=4) 90 87-94  
                 
                 
  • 定性/定量:
    quantitative
  • 研究領域:
    Cardiovascular
  • 引用文獻:
    1. The barberry juice effects on metabolic factors and oxidative stress in patients with type 2 diabetes: A randomized clinical trial , FatemehLazavi.et al,Complementary Therapies in Clinical Practice,2018
    2. Prevention mechanism of 2, 3, 5, 4'-tetrahydroxy-stilbene-2-O-β-D-glucoside on lipid accumulation in steatosis hepatic L-02 cell , Wang W.et al,J Ethnopharmacol. ,2017
    3. A study of serum Apo A-1 and Apo B-100 levels in women with preeclampsia , Timur H.et al,Pregnancy Hypertens.,2016